Is it a 49/50cc scooter? If so, then it isn't permitted on controlled access highways like the 400-Series. The actual list of highways is rather long and can be found at the link below.
http://www.e-laws.gov.on.ca/html/regs/english/elaws_regs_900630_e.htm
From:
R.R.O. 1990, REGULATION 630
1. (1) Subject to subsection (2), no person shall operate,
(a) a bicycle;
(b) a motorcycle having a cylinder swept volume of 50 cubic centimetres or less;
(c) a motorcycle driven by electricity stored in the vehicle;
(c.1) a limited-speed motorcycle, as defined in Ontario Regulation 340/94 (Drivers' Licences) made under the Act;
(c.2) a power-assisted bicycle;
(d) a motor assisted bicycle; or
(e) a wheelchair,
on those controlled-access highways and parts of controlled-access highways described in the Schedule. R.R.O. 1990, Reg. 630, s. 1 (1); O. Reg. 598/05, s. 1; O. Reg. 368/09, s. 1.
(2) Subsection (1) does not apply to a person who resides on lands adjoining the controlled-access highway to which lands there is no other means of access by highway while such person is engaged in gaining access or egress from such lands, and provided that in gaining such access or egress the person proceeds by the shortest route over such controlled-access highway to the lands. R.R.O. 1990, Reg. 630, s. 1 (2).